Secretion Trap Tagging of Secreted and Membrane-Spanning Proteins Using Arabidopsis Gene Traps
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چکیده
منابع مشابه
Secretion trap tagging of secreted and membrane-spanning proteins using Arabidopsis gene traps.
Secreted and membrane-spanning proteins play fundamental roles in plant development but pose challenges for genetic identification and characterization. We describe a "secretion trap" screen for gene trap insertions in genes encoding proteins routed through the secretory pathway. The gene trap transposon encodes a beta-glucuronidase reporter enzyme that is inhibited by N-linked glycosylation sp...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2003
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.103.020099